Kinetics and Structure of a Cold-Adapted Hetero-Octameric ATP Phosphoribosyltransferase
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چکیده
منابع مشابه
A single point mutation leads to an instability of the hetero-octameric structure of yeast phosphofructokinase.
Yeast phosphofructokinase is an oligomeric enzyme whose detectable activity in vitro depends on its hetero-octameric structure. Here we provide data demonstrating that an alanine residue at positions 874 (for the PFK1-encoded alpha-subunit) or 868 (for the PFK2-encoded beta-subunit) is crucial to achieve this structure. Thus subunits carrying substitutions by either aspartate or lysine of this ...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2017
ISSN: 0006-2960,1520-4995
DOI: 10.1021/acs.biochem.6b01138